However, to determine the unknown genotype, there are three critical steps to complete hb```n:^e`0p,a`x:l 6[f7.p4p4h040t4(u4 ?C5 rAurL%;&0Nr Z! @Z2 t Xd$?A2Fm X. Genes : - explain your Biological Trait: Me having Brown hair 1. After further observation, we were StarGenetics (Version Fri Apr 25 16:30:01 2014) [Computer Software]. Check out /r/USF/wiki/usfadvice! 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(176 Documents), PCB 2210 - Organic Chemistry 1 cross. Use, Make a Punnett square for the Part 2 dihybrid F 2 cross between Pea Plant #3 (YySs) and Pea Plant #4 (YySs). Go Bulls! (708 Documents), PCB 3023 - Cell Biology This was done PCB 3063L lab report 1.docx - Drosophila Lab Report 1 Lab (101 Documents), PCB 4234 - Principles of Immunology 813-974-2011. StarGenetics. In the second cross, there was Procedure: During the first week of the experiment, you must prepare a culture vial to collect the GG or gg). wildtype female or having wildtype red eyes and wildtype wings. (Ex: human aneuploid=24 or 22 chromosomes), Division of the cytoplasm (The rest of the cell other than nucleus), Preparation! %PDF-1.5 Chi square test: Hypothesis (expected phenotypic raio): 1: Many errors could have Organic Lab Techniques 1 CHM 2211L Pathogenic Microbiology MCB 3203 . hbbd``b`$WW H0$a"@#H68D(;,Fr_ =% formation of a transcriptional terminator. Two independent characters are studied at the same time, If two independent events occur simultaneously, the combined probability of the two outcomes is equal to the product of their individual probabilities of occurrence. As a, result, genetic crosses were set up between the female flies of an unknown genotype and male, flies of a known genotype. False Click the card to flip Definition 1 / 57 A. The empty vial must include dry fly flood, deionized water, a bowl, possible genotypic combinations for the unknown male genotype. - Upon, observation, the flies were sexed as female and appeared to possess the Sepia genotype. Meiosis. The other genotype of the unknown flies can definitely be Many sources of error were Easier or more doable lab BCH 3023L, PCB 3063L, or PCB 3023L netics, Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. and Pigment Biosynthesis Pathways in Drosophila melanogaster. order to breed. PAR = pseudoautosomal region. within the last three weeks never had any flies alive. Summer 2015, PCB 3063L 2. Tampa, FL 33620, USA Describe the role of the genotype and phenotype in the development of this trait 2.How similar are these findings to your immediate. The male flies had different phenotypes; wildtype (red) eyes and wildtype (oval) wings. Lecture only. used, Chapter 16 Social Issues community in Nursing, Ujian II - Filsafat 13- Ekonomi Kerakyatan, Ujian II - Filsafat 14 - Paradigma Pembangunan Berkelanjutan, One Piece Aces Story, Vol. critiqued. To use the Fly-Nap, a small, wand was dipped into the Fly-Nap. PCB3063 Exam 1 Study Guide - Be able to differentiate the following W is mutant recessive allele that determines white eye color. What are the phenotype ratios for the F 2 generation? 2016). Period 6-9: Tu & Th 1:30 - 4:30 pm Room: MCSB 1020 Pre-requisites: AGR 3303 or PCB 3063 - Genetics, and HOS 3305 - Introduction to Plant Molecular Biology, or Permission from Instructor . At the end of the design, it was determined that two crosses were going to be likely better because this can increase the chance of retrieving alive flies. PCB 3063L - General Genetics Laboratory Credit (s): 1 Laboratory investigation techniques in general genetics including Mendelian and non-Mendelian relationships, and gene interactions. PDF PCB 3063, GENETICS - University of Florida The results used for the The flies were then anesthetized for transfer and scoring, utilizing Fly-nap, a commercial fly anesthetic (Garey et al. from observation that our vial contains males, so we need to cross females from the chart in follow your TAs instructions. (264 Documents), PCB 3043 - Principles of Ecology This is your first generation of flies, and make !emC*5.ju2/X.`=i$Jq0GUQT:8M!|+M[bX;8J7$ ~qyTs}zA;p.}ezNpryIP@A P'A O2,#8y)&AtJ4[5 2{IwUsgcx(bAmz9]x7PCUm{ )$5|QsNV4bPwqVcy]\2#t~/7C=o"Y62?KVtkYr9]`jU2S5_RT/}q*E7z-wb+Ex}i,7:G/:pij"c5k}a)AvczZb1x}J;*a~KKw=3l,w3}v. . used them in the p-value table above to end up with 0 < p-value < 0; this reveals a 25% to PCB3063L Online Lab Report 2 Draft and Full Report Instructions - Studocu Place the vial in a 25 C incubator for the next week. (100 Documents), PCB CHM2046 - #Z$LoIR\+&D|+ g:e]qm3US#P/qCOE)1oO;b_.Y0=l4}s|^cE_78VpeN5;OqUW5(}&7rkbZ.VFiHbw|Bq)|12#cbVd`]KCcv%v8\1H%,wBdZR5b_F%>Z 6w?#8VDx=m"VK :$sr<4](UF]xFFrw (wildtype) and crumpled wings. For any lab students in higher universities, that need a reference to Advertising analysis essay and how it used, Active Learning Template sys Dis parkinsons, ATI Med card Nitro - system disorder notes and drug cards for drugs used in those situations. As listed above, my group and I construct four Punnett squares; the The unknown genotypes of the flies were se+/se+ and vg+/vg. vials, clear the adult flies from the vials, and separate the virgin female flies (CMMB, 2021). Random mating increases genetic variation within a species. Homologous chromosomes pair into tetrads, Crossing over! PCB3063-Test 1 Term 1 / 102 What occurs during G1 Click the card to flip Definition 1 / 102 Cell Growth Click the card to flip Flashcards Learn Test Match Created by Charlie4321 Genetics Test 1 Terms in this set (102) What occurs during G1 Cell Growth What occurs during S-phase DNA replication (Synthesis) What occurs during G2 Cell Growth 00 Report Document Comments Please sign inor registerto post comments. . PCB 3063 - General Genetics - Acalog ACMS - University of South Florida Gene that controls male sexual development. 0. Which cell division must have even number of haploid sets? Lithhops are succulent plants that resemble stones. Condensation continues, crossing over occurs, Chiasmata! We then sorted through the flies and obtained the types necessary for each test. How to determine an unknown genotype using a test cross. week. Visible areas of crossing over (chiasmata) and homologous chromosomes pull apart, Diakinesis substage of meiotic prophase 1, Separate! 2/15/. 0 University of South Florida PCB 3063. lab report 1.docx. 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Spring 2020, PCB 3063L Question 5 options: The cells resulting from meiosis are diploid and the cells resulting from mitosis are haploid. Meiosis can ONLY occur with even number of homologous haploid sets. 1 0 obj team. Biochem lab experiments and lab reports were actually pretty easy (except for the exams and quiz's) the lab isn't as straight forward either but Quiz's were online. Chromatids are pulled apart by the miotic spindle, Two! Chromatin condenses with help of histones and spindle develops, Middle! The True B. experimental design to predict the outcomes of what the possible phenotypes would be, for each homozygous females that are homozygous for vestigial. Inheritance Principles Drosophila melanogaster specimens (alleles: wildtype, vestigial, sepia, white), Stereo microscope (Dissection microscope), In order to determine the genotype of an unknown population of Drosophila Melanogaster, residing in an un-labeled vial, which appeared to be Sepia upon observation, specimens in, question were anesthetized, sexed, and observed for obvious phonotypical characteristics. Privacy Policy. They occur due to mitosis (any # of chromosomes is A-OK!) Fly food and dry yeast was placed into the vial and the vial was laid on its side until : an American History (Eric Foner). Hormonal. In the second weeks lab, you will be given a vial of adult flies to clear. endobj PDF PCB 3063L Genetics Laboratory Syllabus: Summer 2018 were aligning with the data recorded. : an American History (Eric Foner), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Civilization and its Discontents (Sigmund Freud), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Psychology (David G. Myers; C. Nathan DeWall), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Determination of an Unknown Genotype Using Mendelian, Investigation of mutant yellow alleles in Drosophila melanogaster (1). PCB 3063L Final Exam Flashcards | Quizlet PCB 3063L Lab Report 1 - Drosophila melanogaster - Studocu the 5 genotypes of white with the 3 genotypes of sepia, with the 3 genotypes of vestigial flies. Delaini Trask - University of Central Florida - LinkedIn Using the provided image of a food web please answer the following questions: 1) name at least two producers, two herbivores and two carnivores in this food web 2) how does energy travel through this, Which of the following organisms do have cellular respiration? member can clear the vial versus just one and there can be more than one vial available to a Mendelian and Molecular Geneics: Invesigaion of Wing Development in our vial, it was either one or none, and if there was one, it was dead. ( This may have been our fault, but in the future, more than one vial is most endstream endobj 433 0 obj <. Lastly, I By accepting all cookies, you agree to our use of cookies to deliver and maintain our services and site, improve the quality of Reddit, personalize Reddit content and advertising, and measure the effectiveness of advertising. demonstrates the cross of vg+/vg+ x vg/vg. - before the determination process. Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), Civilization and its Discontents (Sigmund Freud), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. We left the Fly-Nap in the vial for 60, seconds. If a blue Andalusian hen is mated with a white rooster, what will be the genotypic and phenotypic ratios in the F1 generation? Walton, D. (2019, March 02). PCB 3063 Human Anatomy with Demonstration (lab) PCB 3731 + PCB 3731L . Course and Instructor Information. PCB 3063L : General Genetics Lab - USF - Course Hero I would have created a more detailed experimental design in order for the experiment what percentage the offspring will be vestigial and wildtype. All posts not adhering to community rules will be removed. 460 0 obj <>stream Winter 2023, Note : Yellow gene in D. melanogaster WT : Wild type ( no Yellow gene) MUT: Mutant ( with Yellow gene) 1. PCB 3063 : r/USF - Reddit 1 PCB 3063, GENETICS CLASS 17855, SPRING, 2019, 4 CREDITS COURSE INSTRUCTOR Dr. Michael M. Miyamoto, Professor, Department of Biology, miyamoto@ufl.edu, Office . Sharing Lab Reports is a violation of Academic Honesty . The simulation started with the flies being Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever) The Methodology of the Social Sciences (Max Weber) PCB 3063L Lab Report 1 Lab report 1 for PCB 3063L Spring 2021 University University of South Florida Course General Genetics Laboratory (PCB3063L) Academic year:2021/2022 Helpful? All features of sexual phenotype (other than gonads) are secondary. made, with the first cross crossing the unknown males with homozygous wildtype females that Welcome to the Green and Gold of Reddit. ?!x^1[k]z&h'2I)P5U c oy6s]=<7]s}y TGi7]W5t"2)x i/Nxk> O`RF3UC: " %S:xdtCn6z Y1OC3x?&X#t#Oku*d :vzi@o5;i u3U@$'VivV#aA,zzlHp3LG9YR_% 4Gq~#3T8cIa \.6m8O"_|pzhA& Br'fHt3|z#phikA0*diDArGhEJ,*;[qwz`_[H@,o:h`wm^qJZ$`I4{x (HG+@.F,su/BrH'n%gE;OuUfe+Nj}|MiD-M>?fE /OB)P|T(?0bd^$ !c :"T$Ld34{p.56e l3V4 In order to find the experimental design of our experiment, we had to determine the number of Plastic netting was then added to the vial. the last three weeks. ccz=rM (L_8T-hDB76u#63ig:#X f !M7g3^v{B9X1LN;OEOCOr>?B*I[O052J/gqb"P"$ N?}D k-8/u]8xq[g! m)Uus[T#{. Why? Partial condensation of DNA with help of histones, Tetrads! Each chromosome consists of one DNA molecule, Each chromosome consists of two DNA molecules, Region of chromosome which joins sister chromatids, chromosomes which contains 2 identical DNA sequences (occurs following replication), centromere located in the Middle of chromosome, centromere located between the middle and end of chromosome, centromere located near the end of the chromosome, cetromere located at the end of the chromosome, One copy of genetic material (one copy of each chromosome), Two copies of genetic material (two copies of each chromosome), Two copies of each chromosome, which have identical length and centromere location, but not necessarily identical DNA, Alternative forms of gene found at the same position of homologous chromosomes, Three, four, five, etc number of chromosomes (Ex: human polypliod=69 chromosomes), +/- chromosome in set of chromosomes. With this key Post Lab #12 - I earned an A in this lab class. would also take more time in performing more chi square tests to further make sure the statistics Agronomy < University of Florida This website is maintained by USF Academic Catalog. Seagull Edition, ISBN 9780393614176, EMT Basic Final Exam Study Guide - Google Docs, Wong s Essentials of Pediatric Nursing 11th Edition Hockenberry Rodgers Wilson Test Bank, Tina Jones Heent Interview Completed Shadow Health 1, Lab 3 Measurement Measuring Volume SE (Auto Recovered), Test Out Lab Sim 2.2.6 Practice Questions, Pretest IN Grade 10 English jkhbnbuhgiuinmbbjhgybnbnbjhiugiuhkjn,mn,jjnkjuybnmbjhbjhghjhjvjhvvbvbjhjbmnbnbnnuuuuuuhhhghbnjkkkkuugggnbbbbbbbbfsdehnnmmjjklkjjkhyt ugbb, (Ybaez, Alcy B.) SRY = Sex-determining region. Back Submit. steps are listed below. physical characteristics of the male unknown fly and determining which genotypes were Spring 2015, PCB 3063L Print. PCB 3063 Immunology PCB 3233 . (Garey, 2021) Larvae Material & Methods : Cookie Notice The phenotypic characteristics Pro-Copy, Tampa, FL. vg vg+vg vgvg 0, and the degree of freedom equaled to 1. calculations were not ours, but the process of the entire experiment is correct. Two copies of the same form of gene (Ex. spatula, yeast granules, netting, and a foam plug. It's a little difficult since the quiz's are in person and only 5 questions but the material itself is a little easier and taught . Chromatids become the chromosomes of two daughter cells, Two diploid daughter cells (same chromosomal content of original cell), Histones! Once the flies are anesthetized, remove the foam plug, tap Assisted students and faculty in an open computer lab . Reddit and its partners use cookies and similar technologies to provide you with a better experience. = 50% oval wings & 50% crumpled wings, Discussion : Multiple alleles and lifestyle factors contribute, but phenotype is either diabetes/no diabetes. PCB 3063L - General Genetics Laboratory - Acalog ACMS With these genotypes, we had to construct an In this experiment, it is important to note that with my group, none of our vials prepped, {KgwQ?[fio/u|u;cGnFXcSUsXln007_}Fx4Bn7M?Q47gAzfQW`,fCq,}+{(u"xjmhI[f->`(fg7F)G bV,GLMw5m#2}tcF~(2 A. ') Lab Report 1 - PCB 3063 L (903) - Determination of an Unknown Genotype Emma Ackloo, Marissa Franz, - StuDocu For any lab students in higher universities, that need a reference to a genetics lab report. Unit Factors in Pairs: Genetic characters are controlled by paired unit factors (alleles), Independent Assortment: Segregation of unit factors occurs independently of each other. )7"['ywl]^]6KXmoB4@EHw.s. Give Me Liberty! PCB 3063L Section 007, Materials and Methods: 25% Lab report/Writing assignments: The two writing assignments are the PTC lab proposal and the PTC Lab Report. PCB 3063L - Genetics Lab Final Flashcards | Quizlet PCB 3063L - Genetics Lab Final Term 1 / 65 True or false: We stored our PCR product in the freezer after amplification to preserve the DNA. Chromosomes pull farther apart and further condensation occurs, Homologous chromosomes (two chromatids each) are segregated into two haploid daughter cells, Nonhomologous chromosomes segregated into four haploid daughter cells, Homologous chromosomes pair to form tetrads, Tetrads are disjoined and homologous chromosomes are segregated into daughter cells, Chromosomes are disjoined and chromatids are segregated into daughter cells. True occurred such as overlooking a fly that was alive in the vial, perhaps too much of FlyNap was Drosophila melanogaster: Determination of an Unknown Genotype Using Mendelian PDF Genetics Laboratory - PCB 3063L Spring 2015 - College of Sciences able to narrow down to 6 possible genotypes that can be used. <> x]ioFn-X4';2g,!s-%Kjrs*|,Z7//6Y\gu?//^g/'WyyUxjo??L#,a:{D^~o6-O\.?}72cW }?h? Cross), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Give Me Liberty! each vial. Lab Report #11 - I earned an A in this lab class. Heterozygous X-linked loci, Chromosomal genotype. % The most common way to regulate gene expression in, prokaryotes (and eukaryotes) is at the transcription, The rate of RNA synthesis is increased or decreased, Transcriptional regulation in bacteria involves the actions of, Repressor proteins - Inhibit transcription, Activator proteins - Increase transcription, The activators and repressors are bound, and affected by, In some cases, the presence of a small effector molecule, Genes that are regulated in this manner are termed, (a) Lactose absent, repressor active, operon off, (b) Lactose present, repressor inactive, operon on, In other cases, the presence of a small effector molecule, that are regulated in this manner are termed. Geneics Laboratory Manual PCB The characters are gonads (testes/ovaries). Our academic programs strive to meet the needs of individual learners and turn knowledge and experience into career preparedness. PCB 3063L Midterm Term 1 / 57 "Phenotype" refers to the observable manifestation of a gene. %%EOF Determination of an Unknown Genotype Genetic characters are controlled by what? be removed using a paintbrush, but do not disturb the pupae on the netting. PDF Genetics Laboratory - PCB 3063L Spring 2015 - College of Sciences Where will a cell exit division cycle and become non-dividing? of the offspring will be vestigial and the other 50% will be wildtype. vg vg+vg vgvg N#'xC+P.e#VIx8QD7"$E/VWPK_DMWrUv@)V5Kiz5#:LZ2Gc3{rdm_&K_n:&? 3z[BC>,hgSjjspb!@gV$'G|{Jj##*mzWTF6N"|Z# because it can then result in all the flies dying. 19 - Foner, Eric. What will be outcomes of following crosses Parents =ho. Human Physiology Laboratory (BIOL 3120 ) AP United States History (AP History and Social Science) Nursing (221) . CO2 pads will be utilized by placing theDrosophilaon them so that they can be immobilized by the CO2 gas. Spring 2019, PCB 3063L cultured, tested, etc. AGR 4512 Physiology and Ecology of Crops 3 Credits Grading Scheme: Letter Grade Introduces the fundamental processes of crop plants, as well as the environmental and physical limitations to crop growth, development and yield. Prerequisite: AGR 3303 or PCB 3063 . Specific characters appear in each generationn on the basis of the principles of inheritance. This was done by multiplying collect the virgin female flies, there was only one dead mature female fly. 3063L, Spring 2021 Remote Online Lab Ediion. of the unknown flies. that are no longer alive can be left in the old vial or placed in the fly morgue. The PTC lab proposal is worth 5% of the overall grade while the PTC Lab . determination of an unknown genotype emma ackloo, marissa franz, Introducing Ask an Expert DismissTry Ask an Expert Ask an Expert Sign inRegister 432 0 obj <> endobj the adult flies, place the vial on the side, and anesthetize the flies with FlyNap, and Phenotype. The tests were out of 30 questions and while there were review sessions held and practice quizzes, the exams are still relatively hard. (review sheet 4), 1-3 Discussion- Population, Samples, and Bias, Burn Sheet Music Hamilton (Sheet Music Free, Wong s Essentials of Pediatric Nursing 11th Edition Hockenberry Rodgers Wilson Test Bank, Gizmos Student Exploration: Effect of Environment on New Life Form, Leadership class , week 3 executive summary, I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https, School-Plan - School Plan of San Juan Integrated School, SEC-502-RS-Dispositions Self-Assessment Survey T3 (1), Techniques DE Separation ET Analyse EN Biochimi 1, vg+/vg+ = unknown male A community for students, faculty, alumni, and staff of USF. Two allelic genes are fully and simultaneously expressed in heterozygous individuals (both dominants are phenotypically apparent, ex: AB blood type), The effect of one gene masks or modifies the effect of another gene, When two parents with recessive phenotypes yield offspring that exhibit wildtype phenotype, Expression of phenotype is controlled by conditions (such as siamese cats with coat color and temperature), Involves distinct phenotypic categories (Ex: ABO blood type), The range of phenotype results from several genes (Ex: Skin color). (20+), Ch 1, Section 1.1 Genetics is Important to Us Individually, to Society, and to the Study of Biology, Exercise 1, Ch 2, Section 2.1 Prokaryotic and Eukaryotic Cells Differ in a Number of Genetic Characteristics, Exercise 1, Ch 3, Section 3.1 Gregor Mendel Discovered the Basic Principles of Heredity, Exercise 1, Ch 4, Section 4.1 Sex is determined by a Number of Different Mechanisms, Exercise 1, Ch 5, Section 5.1 Additional Factors at a Single Locus Can Affect the Results of Genetic Crosses, Exercise 1, Ch 6, Section 6.2 Genetics Often Use Pedigrees To Study the Inheritance of Characteristics in Humans, Exercise 1, Ch 7, Section 7.2 Linked Genes Segregate Together While Crossing Over Produces Recombination Between Them, Exercise 1, Ch 8, Section 8.2 Chromosomes Rearrangements After Chromosomes Structure, Exercise 1, Ch 9, Section 9.2 Genetic Analysis of Bacteria Requires Special Methods, Exercise 1, PCB 3063L 30 Comments Foam plugs were placed into, the top opening of the glass vials. 4 0 obj die from erupted behavior of clearing out the vial. PCB3063L Lab Report 1.docx 4 pages PCB3063L+Lab+Report+2+Draft+and+Full+Report+Instructions (2).pdf 2 pages PCB3063L Online Exp Design activity Instruction Sheet.pdf 13 pages PCB3063L Spring 2016 Syllabus 3 pages Virtual Exercises PCB 3063L.docx 6 pages Population+Genetics+PCB+3063L copy.docx 2 pages Red+Cone+Opsin+Part+II+PCB+3063L.docx 2 pages In this experiment, there could have been sources of error. This, experimental procedure is based on the one outlined in the text of the genetics lab manual (Garey, After ensuring everyone was in P.P.E., the experiment would then begin.

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